Rotor-Gene Q MDx 5plex HRM Platform

Features Optimal thermal and optical performance due to rotary format Robust technology, requiring minimal maintenance Easy-to-use software with advanced security features Product Details The Rotor-Gene Q MDx instrument, with Rotor-Gene Q software version 2.1.0 or higher, is a real-time nucleic acid amplification and detection system, which measures nucleic acid signals from amplified DNA using fluorescent detection. Principle Unique rotary design for outstanding performance The Rotor-Gene Q MDx uses a sophisticated heating and cooling design to achieve optimal reaction conditions. The unique rotary format ensures optimal thermal and optical uniformity between samples, which is critical for precise and reliable analysis (see figure " Cross-section of the Rotor-Gene Q MDx"). Each tube spins in a chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling. Detection is similarly uniform. When each tube aligns with the detection optics, the sample is illuminated and the fluorescent signal is rapidly collected from a single, short optical pathway. This thermal and optical uniformity results in sensitive, precise, and fast real-time PCR analysis. Robust optics for multiplex applications With a choice of up to 6 excitation sources and 6 detection filters (the red and HRM channels are not intended for use with FDA cleared or approved nucleic acid tests), combined with a short, fixed optical path, the Rotor-Gene Q MDx can be used for multiplex reactions, ensuring minimum fluorescence variability between samples and eliminating the need for calibration or compensation. Samples are excited from the bottom of the chamber by a light-emitting diode (see figure " Cross-section of the Rotor-Gene Q MDx"). Energy is transmitted through the thin walls at the base of the tube. Emitted fluorescence passes through emission filters on the side of the chamber and is then collected by a photomultiplier. The fixed optical path ensures consistent excitation for every sample, which means that there is no need to use a passive internal reference dye such as ROX.