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Qiacuity One Plex

<p>The QIAcuity Digital PCR System is designed to deliver precise and multiplexed quantification results for mutation detection, copy number variation (CNV), gene expression studies, gene-editing analysis, and many more. This nanoplate-based system seamlessly integrates a standard dPCR workflow of partitioning, thermocycling and imaging into a walk-away automated platform with minimal hands-on time.</p>
<h4 class="section">Features</h4>
<section>
<div class="rich-text product-description">
<ul>
<li>Fully integrated system</li>
<li>Scalable format (1-, 4- and 8-plate instruments)</li>
<li>Advanced multiplexing capabilities (up to 12 plex)</li>
<li>Up to six standard channels plus two hybrid channels for LSS dyes</li>
<li>Flexible sample throughput</li>
<li>Comprehensive results in approx. 2 hours</li>
</ul>
<h4 class="section">Performance</h4>
<section>
<div class="rich-text product-description">
<p>The QIAcuity Digital PCR System makes absolute quantification accessible and affordable for all laboratories. The walk-away automation integrates and streamlines the entire digital PCR workflow of partitioning, thermocycling and imaging into a single instrument with minimal hands-on time. It is also easy to adapt your current qPCR assays to the QIAcuity Digital PCR System. No change in plate handling is required when coming from qPCR, assuring fast assay setup and quick results in approx. 2 hours.</p>
<h4 class="section">Principle</h4>
<section>
<div class="rich-text product-description">
<p>In just 3 simple steps, you can have the dPCR result you want in approx. 2 hours: pipette and load, run the experiment, analyze results.</p>
<h4 class="section">Procedure</h4>
<section>
<div class="rich-text product-description">
<p>Just like in qPCR experiments, sample preparation includes the transfer of master mix, probes and primers to a 96- or 24-well nanoplate, followed by the addition of samples. The system integrates partitioning, thermocycling and imaging into a single fully automated instrument that takes users from the sample to result in under 2 hours. One can perform analysis on the Software Suite, providing the concentration in copies per microliter of your target sequence as well as for quality control such as positive samples or NTC. This analysis can also be extended to remote computers within the same local area network (LAN).</p>
</div>
</section>
<h4 class="section">Applications</h4>
<section>
<div class="rich-text product-description">
<p>The QIAcuity instruments, together with the QIAcuity Nanoplates and the QIAcuity PCR kits, enable digital PCR applications, including:</p>
<ul>
<li>Rare mutation detection</li>
<li>Copy number variation analysis</li>
<li>Gene expression analysis</li>
<li>Pathogen detection</li>
<li>Genotyping</li>
<li>miRNA research</li>
<li>Cell and gene therapy</li>
</ul>
</div>
</section>
<h4 class="section">Software</h4>
<section>
<div class="rich-text product-description">
<p>The QIAcuity Software Suite provided with the instrument and installed on a separate computer controls one or multiple QIAcuity instruments, either connected directly to one instrument or using an existing local area network (LAN). Using the QIAcuity Software Suite, digital PCR experiments, samples and reaction mixes can be defined, assigned to Nanoplates, and transferred to the QIAcuity instrument. After the run, data can be analyzed, reports can be created and data can be exported for external analysis. The software provides multiple template functionalities to make repeating plate layouts or plate run parameters easily accessible, further transforming your digital PCR experience.</p>
<p>When integrated into a local area network, the computer is hosting the QIAcuity Software Suite function as a server that is accessible via LAN to other computers serving as clients. This enables multiple users to access the software from other rooms or offices and analyze data via a standard browser without the need to install the software on multiple computers or access and exchange data via internet connections.</p>
</div>
</section>
</div>
</section>
<p>&nbsp;</p>
</div>
</section>
</div>
</section>

Qiacuity Digital PCR System

QIAcuity One 2Plex Digital PCR System

Qiacuity Brochure

One-plate digital PCR instrument (cat.no 911000 QIAcuity One, 2plex instrument) for detecting up to 2 fluorescent dyes, roller, USB flash memory and QIAcuity Software

QIAcuity One, 5plex Digital PCR Instrument

QIAquant 96 5plex (230 V) Real Time PCR Instrument

QIAxcel Connect

QIAgility HEPA / UV (incl.Laptop)

Rotor-Gene Q MDx 5plex HRM Platform

Qiagen Digital PCR System

Qiacuity One 5plex digital pcr

Qiacuity brochure

One-plate digital PCR instrument (cat.no 911020 QIAcuity One, 5plex instrument) for detecting up to 5 fluorescent dyes or up to 8 fluorescent dyes operating under software version 3.1 or higher, enabling up to 12plex analysis, roller, USB flash memory and QIAcuity Software Suite

Qiaquant 5Plex

<p>Features Fast cycling protocol, down to 30 minutes for 40 cycles Multiplex detection of up to 5 different targets Temperature ramping rates up to 8°C/s Temperature uniformity down to ±0.15°C across the sample block Gradient function for assay optimization Product Details QIAquant instruments are all-round systems designed for performance. They combine high-quality thermal elements for fast heating and cooling ramping rates and a fast multi-channel detection system for shorter cycling times. The open system is compatible with plasticware in standard SBS formats, low-and normal-profile, and all skirting types. Visit the QIAquant consumables page for qPCR plates and foils. Performance The QIAquant qPCR instruments deliver on the fundamental expectations to combine high performance optical detection of qPCR products with a high performance thermal block. The result is an all-round versatile system for quantitative PCR applications such as expression analyses, genotyping and the detection of pathogens. The combination of high quality thermal elements, fast heating and cooling ramping rates and the fiber-optic shuttle system enables shorter cycling times down to less than 30 minutes for 40 cycles.</p>

QIAquant 96 5plex (230 V) - High performance real-time PCR thermal cycler with 5plex detection system, 96-well block, software, and touchscreen interface.

Qiaquant Brochure

Manufacturer: QIAGEN GmbH                    | 

Qiaexcel Connect

<p>Features Ready-to-use gel cartridges High sensitivity, high resolution Process up to 96 samples without manual intervention Product Details Fully automate the fragment analysis and quality control of up to 96 samples per run with QIAxcel Connect. This capillary-electrophoresis instrument uses ready-to-run gel cartridges that eliminate tedious gel or consumable preparations. It also reduces handling errors. Various QIAxcel Kits meet your specific needs (see High versatility with multiple kits): High-resolution genotyping Fast single or multiplex PCR screening QC of cell-free DNA NGS library QC Fast single-amplicon analysis, such as HLA typing RNA quality checks And if you want to monitor your runs from outside the lab, you can connect to the optional QIAsphere system on your instrument and phone. See figures High versatility with multiple kits. High versatility with multiple kits. Principle QIAxcel Connect uses prefilled gel cartridges to perform automated capillary electrophoresis. Simply place samples and buffers into the instrument and start the run. The tips of the gel cartridges move into the sample wells. An electric field creates a positive charge. This makes the negatively charged nucleic acid fragments migrate through the gel in the capillaries, with the smaller fragments travelling faster than the larger ones. Within the capillary, a dye intercalates into the nucleic acid. As the fragments pass a detector, it measures the signals emitted by the dye. The QIAxcel ScreenGel software converts these signals to digital data; you can see them in real time as electropherograms or gel images (see Cartridge-based gel electrophoresis). See figures Cartridge-based gel electrophoresis. Cartridge-based gel electrophoresis. Procedure Turn on the instrument and put in the buffer tray filled with buffer and markers. Insert the QIAxcel Gel Cartridge and your samples into the instrument. Select your analysis protocol and run the samples. See your results in real time, displayed on your QIAxcel ScreenGel user interface (see Plug-and-play electrophoresis). See figures Plug-and-play electrophoresis. Plug-and-play electrophoresis. Applications QIAxcel Connect is highly suitable for analysis and quality control of: Next-generation sequencing (NGS) libraries Low-concentration samples, such as liquid biopsy Degraded samples, such as formalin-fixed paraffin-embedded (FFPE) tissue Multiplex PCR products with high-resolution requirements Software The QIAxcel ScreenGel software runs on the laptop that comes with your QIAxcel Connect. It uses a system wizard to make experiment setup and run checks easy. Then it displays results according to user preference, whether individually or in overlay view for easy comparison. It can also display multiple peak properties (such as height and width of each peak, and number of peaks) in table format. All this data can be readily saved and exported from the software, making it easier to generate reports. To get objective measurements and easily determine RNA quality and integrity, the QIAxcel ScreenGel software uses the RNA Integrity Score (RIS) system. RIS has a high correlation with RIN (R2=0.9229) (see Correlation of RIN and RIS) but is more reliable in predicting qRT-PCR assay outcomes when RNA is degraded, for instance, by UV or formalin.</p>

QIAxcel Connect - Effortless, cost-effective, high-resolution DNA or RNA gel electrophoresis – all in a single instrument

Qiagility

<p>Features Automated PCR setup in all formats Convenient, easy-to-use software Pre-optimized experiment setup Standardized results and increased productivity Elimination of manual pipetting steps Product Details The QIAgility is a compact benchtop instrument that enables rapid, high-precision setup of PCR experiments with ready-to-use software for ease and convenience. The software provides step-by-step guidance for worktable setup and automatic calculation of all mixes, eliminating the need to program pipetting steps. Just select the kit and cycler, define your targets and start the run. For added flexibility the QIAgility is operated via a laptop computer. Explore the virtual demo to learn more about the QIAgility. ​QIAgility operates in conjunction with QIAgility Accessories and QIAgility Consumables. Performance The QIAgility fully automates proven QIAGEN PCR kits for use with various workflows, and offers pure pipetting precision, enhanced safety features, and exceptional reliability and reproducibility. Unmatched pipetting precision with QIAgility The QIAgility instrument operated by the QIAgility Setup Manager Software demonstrates exceedingly high accuracy and precision of dispensed volumes as verified by the Artel MVS system (see figure “ Exceptional pipetting precision and accuracy spanning over a broad volume range”). These results indicate that the QIAgility can produce automated, high-performance liquid dispensing results, while avoiding common errors that can be associated with manual pipetting and thereby reducing the time to result. Regardless of whether you pipet water or QIAGEN ready-made reaction mix to set up a PCR experiment, the QIAgility instrument can dispense volumes that are highly precise and also generate significantly reproducible replicates (see figure “ Unrivaled pipetting precision and reproducibility of the QIAgility for master mix solutions”). High performance PCR setup using QIAgility A QuantiNova SYBR Green PCR assay setup was performed manually and automated in parallel using the QIAgility along with its Setup Manager Software. The amplification run was done on the Rotor-Gene Q and resulting data analyzed using the Q-Rex Software. Consistent results were obtained from both manual and automated reaction setup using the same template dilutions (see figure “ Comparison of manual and automated assay setup using series dilutions”). Exceptional linearity of QIAgility QIAgility exhibits excellent linearity and assures accurate and reliable use of sample for an assay. To assess the linearity of the system, pure gDNA was serially diluted using the QIAgility and measured for their concentrations on the QIAxpert. The quantifications from the QIAxpert were plotted against the known concentration values, and the resulting plot demonstrates the exceptional linearity (with a regression coefficient of &gt;0.999) of QIAgility (see figure “ High system linearity of QIAgility”). See figures Exceptional pipetting precision and accuracy spanning over a broad volume range Exceptional pipetting precision and accuracy spanning over a broad volume range Unrivaled pipetting precision and reproducibility of the QIAgility for master mix solutions Unrivaled pipetting precision and reproducibility of the QIAgility for master mix solutions Comparison of manual and automated assay setup using series dilutions Comparison of manual and automated assay setup using series dilutions High system linearity of QIAgility High system linearity of QIAgility Principle Automated PCR setup is rapid and reliable and eliminates manual pipetting steps that are typically prone to human error. Liquid-handling tasks are performed by the single-channel pipetting head, which provides fast transfer of liquids with various types of plasticware. For example, 96 samples with the QuantiFast SYBR Green PCR Kit with 2 standard series can be setup in approximately 45 minutes. The worktable features a flexible layout and supports almost all kinds of cyclers and labware, giving high application versatility. Dynamic liquid-level sensing, facilitated by conductive filter tips offers maximum safety control. The advanced design of the QIAgility delivers improved process safety, giving you peace of mind. A built-in sensor ensures that the instrument pauses when the hood is opened during a run, protecting users from moving parts. The UV light ensures effective worktable decontamination and the HEPA filter provides positive clean air pressure throughout PCR setup. QIAgility is a compact benchtop instrument with a very small footprint. It fits easily onto most lab benches and can be readily moved into biosafety level laboratories. For added flexibility, the QIAgility comes with a laptop computer. To reduce your work load, comprehensive run reports containing all run information are generated automatically during each protocol</p>

QIAgility HEPA / UV (incl.Laptop) - Robotic workstation for automated PCR setup (with UV light and HEPA filter), comes with QIAgility software

Rotorgene

<p>Features Optimal thermal and optical performance due to rotary format Robust technology, requiring minimal maintenance Easy-to-use software with advanced security features Product Details The Rotor-Gene Q MDx instrument, with Rotor-Gene Q software version 2.1.0 or higher, is a real-time nucleic acid amplification and detection system, which measures nucleic acid signals from amplified DNA using fluorescent detection. Principle Unique rotary design for outstanding performance The Rotor-Gene Q MDx uses a sophisticated heating and cooling design to achieve optimal reaction conditions. The unique rotary format ensures optimal thermal and optical uniformity between samples, which is critical for precise and reliable analysis (see figure " Cross-section of the Rotor-Gene Q MDx"). Each tube spins in a chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling. Detection is similarly uniform. When each tube aligns with the detection optics, the sample is illuminated and the fluorescent signal is rapidly collected from a single, short optical pathway. This thermal and optical uniformity results in sensitive, precise, and fast real-time PCR analysis. Robust optics for multiplex applications With a choice of up to 6 excitation sources and 6 detection filters (the red and HRM channels are not intended for use with FDA cleared or approved nucleic acid tests), combined with a short, fixed optical path, the Rotor-Gene Q MDx can be used for multiplex reactions, ensuring minimum fluorescence variability between samples and eliminating the need for calibration or compensation. Samples are excited from the bottom of the chamber by a light-emitting diode (see figure " Cross-section of the Rotor-Gene Q MDx"). Energy is transmitted through the thin walls at the base of the tube. Emitted fluorescence passes through emission filters on the side of the chamber and is then collected by a photomultiplier. The fixed optical path ensures consistent excitation for every sample, which means that there is no need to use a passive internal reference dye such as ROX.</p>

Rotor-Gene Q MDx 5plex HRM Platform - Real-time PCR cycler with 6 channels (the red and HRM channels are not intended for use with FDA cleared or approved nucleic acid tests), laptop computer, software, accessories

<p>Rotor-Disc Heat Sealing Film (60 films)</p>

Rotor-Disc Heat Sealing Film (60 films)

Rotor-Disc 72 (24 individually wrapped discs)

Reagent Bottles, 30 ml (6)

Filter-Tips, 1000  l, wide-bore (1024 tips)

Filter-Tips, 50  l (960 tips)

Sample Tubes CB (2ml)

QuantiNova SYBR Green PCR Kit (500 reactions)

QIAcuity Probe PCR Kit (1mL)

QIAGEN Plasmid Maxi Kit (10 preps) [PEX 24] [Discounted Price - While stock last]

Nuclease-Free Water (1000 ml)

RNeasy Protect Animal Blood Kit (50 preps) [PEX 9]

QIAamp DNA Mini Kit (50 preps) [PEX 12] (Discounted Price - While stock last)

Buffer RWT (concentrate, 80 ml) [PEX 9]

RNeasy Plus Mini Kit (250 preps) [PEX 9]

mericon DNA Bacteria Plus Kit (50 preps) [PEX 12]

Nuclease-Free Water (5 Liter)

QuantiNova Probe RT-PCR Kit (100 reactions)

<p>Rotor-Disc 72 (24 individually wrapped discs)</p>

<p>Filter-Tips, 1000 l, wide-bore (1024 tips)</p>

<p>QuantiNova SYBR Green PCR Kit (500 reactions)</p>

<p>QIAGEN Plasmid Maxi Kit (10 preps) [PEX 24] [Discounted Price - While stock last]</p>

<p>RNeasy Protect Animal Blood Kit (50 preps) [PEX 9]</p>

<p>QIAamp DNA Mini Kit (50 preps) [PEX 12] (Discounted Price - While stock last)</p>

<p>Buffer RWT (concentrate, 80 ml) [PEX 9]</p>

<p>RNeasy Plus Mini Kit (250 preps) [PEX 9]</p>

<p>mericon DNA Bacteria Plus Kit (50 preps) [PEX 12]</p>

<p>QuantiNova Probe RT-PCR Kit (100 reactions)</p>
<p>Expiry date : 17 Apr 2026</p>

QuantiNova Probe RT-PCR Kit (100 reactions)

Expiry date : 17 Apr 2026