Use ekoTAQ PLUS GREEN 2X Master Mix when the PCR products will be analysed by agarose gel electrophoresis and ethidium bromide staining.
Key Features:
- Outstanding performance and value, perfect for routine PCR.
- Can be cycled up to 99 °C to amplify the most challenging GC-rich templates others cannot.
- Ready-to-use PCR reaction master mixes, contain dNTPs and PCR Enhancer.
- Non-proofreading polymerase
- ekoTAQ PLUS GREEN contains tracking dyes for gel electrophoresis
Product information
More effective than other Taq master mixes. ekoTAQ PLUS and PLUS GREEN 2X Master Mixes contain a proprietary PCR enhancer, so they can successfully amplify templates that fail to amplify with other PCR master mixes (Figure 1). High density reaction buffer enables direct gel loading following PCR.
Excellent results in routine PCR. ekoTAQ PLUS GREEN contain high purity, high activity ekoTAQ DNA Polymerase for reliable amplification of templates up to 5 kb (Figure 1).
Amplify challenging GC-rich templates. These 2X Master Mixes can be cycled up to 98 °C to amplify challenging GC-rich templates others cannot (Figure 2).
Convenient tracking dyes in ekoTAQ PLUS GREEN. On a 1% agarose gel, the blue dye migrates at the same rate as a 5 kb DNA fragment, and the yellow dye migrates at 75 bp. See Figure 3. If desired, the dyes are easily removed by standard DNA purification products or ethanol precipitation. For fluorescence or absorbance detection assays, ekoTAQ PLUS 2X Master Mix offers the same great performance without the dyes.
Flexible & easy to use. ekoTAQ PLUS GREEN are packaged in vials that can be stored in the refrigerator (+4 °C) for up to 3 months. They are stable for at least 10 freeze-thaw cycles.